Selective gene amplification.
نویسندگان
چکیده
We describe a system for directed evolution based on in vitro compartmentalisation in which amplification of a gene is coupled to the formation of product by the enzyme it encodes. This approach mimics the process of natural selection; 'fitter' genes--encoding more efficient enzymes--have more 'offspring'. It allows selection for any activity so long as a product-specific ligand (e.g. an antibody) is available.
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ورودعنوان ژورنال:
- Protein engineering, design & selection : PEDS
دوره 20 12 شماره
صفحات -
تاریخ انتشار 2007